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Dosing is once daily, subcutaneously, with the timing chosen to align with natural lipolytic windows, typically before bed (to coincide with overnight fasting and growth hormone pulses) or first thing in the morning before training and food

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doi: 10.3233/CH-242134 56 BullFCAl-AnsariSSBiddleSBorodulinKBumanMPCardonGet al

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The following day, cells were transfected with 20 nM of siRNA targeting CD36 (sc-29995) from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, Texas, USA) using Lipofectamine RNAiMAX Reagent from Thermo Fisher Scientific in Opti-MEM media from Gibco accordingly to manufacturers instructions for 48 h, refreshed every day before being treated or not with PA (200 M) during 24 h